发表状态 | 已发表Published |
题名 | A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid |
作者 | |
发表日期 | 2023-12-01 |
发表期刊 | Nature Communications
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卷号 | 14期号:1 |
摘要 | Synthetic auxotrophy in which cell viability depends on the presence of an unnatural amino acid (unAA) provides a powerful strategy to restrict unwanted propagation of genetically modified organisms (GMOs) in open environments and potentially prevent industrial espionage. Here, we describe a generic approach for robust biocontainment of budding yeast dependent on unAA. By understanding escape mechanisms, we specifically optimize our strategies by introducing designed “immunity” to the generation of amber-suppressor tRNAs and developing the transcriptional- and translational-based biocontainment switch. We further develop a fitness-oriented screening method to easily obtain multiplex safeguard strains that exhibit robust growth and undetectable escape frequency (<~10) on solid media for 14 days. Finally, we show that employing our multiplex safeguard system could restrict the proliferation of strains of interest in a real fermentation scenario, highlighting the great potential of our yeast biocontainment strategy to protect the industrial proprietary strains. |
DOI | 10.1038/s41467-023-42358-4 |
URL | 查看来源 |
收录类别 | SCIE |
语种 | 英语English |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:001095513800016 |
Scopus入藏号 | 2-s2.0-85174228354 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://repository.uic.edu.cn/handle/39GCC9TT/11091 |
专题 | 北师香港浸会大学 |
通讯作者 | Shen, Yue |
作者单位 | 1.College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049,China 2.BGI Research,Shenzhen,518083,China 3.BGI Research,Changzhou,213299,China 4.Guangdong Provincial Key Laboratory of Genome Read and Write,BGI Research,Shenzhen,518083,China 5.Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science,BNU-HKBU United International College,Zhuhai,519087,China 6.BNU-HKBU United International College,Zhuhai,519087,China |
推荐引用方式 GB/T 7714 | Chang, Tiantian,Ding, Weichao,Yan, Shiruiet al. A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid[J]. Nature Communications, 2023, 14(1). |
APA | Chang, Tiantian., Ding, Weichao., Yan, Shirui., Wang, Yun., Zhang, Haoling., .. & Fu, Xian. (2023). A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid. Nature Communications, 14(1). |
MLA | Chang, Tiantian,et al."A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid". Nature Communications 14.1(2023). |
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