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题名A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid
作者
发表日期2023-12-01
发表期刊Nature Communications
卷号14期号:1
摘要

Synthetic auxotrophy in which cell viability depends on the presence of an unnatural amino acid (unAA) provides a powerful strategy to restrict unwanted propagation of genetically modified organisms (GMOs) in open environments and potentially prevent industrial espionage. Here, we describe a generic approach for robust biocontainment of budding yeast dependent on unAA. By understanding escape mechanisms, we specifically optimize our strategies by introducing designed “immunity” to the generation of amber-suppressor tRNAs and developing the transcriptional- and translational-based biocontainment switch. We further develop a fitness-oriented screening method to easily obtain multiplex safeguard strains that exhibit robust growth and undetectable escape frequency (<~10) on solid media for 14 days. Finally, we show that employing our multiplex safeguard system could restrict the proliferation of strains of interest in a real fermentation scenario, highlighting the great potential of our yeast biocontainment strategy to protect the industrial proprietary strains.

DOI10.1038/s41467-023-42358-4
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收录类别SCIE
语种英语English
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:001095513800016
Scopus入藏号2-s2.0-85174228354
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://repository.uic.edu.cn/handle/39GCC9TT/11091
专题北师香港浸会大学
通讯作者Shen, Yue
作者单位
1.College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049,China
2.BGI Research,Shenzhen,518083,China
3.BGI Research,Changzhou,213299,China
4.Guangdong Provincial Key Laboratory of Genome Read and Write,BGI Research,Shenzhen,518083,China
5.Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science,BNU-HKBU United International College,Zhuhai,519087,China
6.BNU-HKBU United International College,Zhuhai,519087,China
推荐引用方式
GB/T 7714
Chang, Tiantian,Ding, Weichao,Yan, Shiruiet al. A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid[J]. Nature Communications, 2023, 14(1).
APA Chang, Tiantian., Ding, Weichao., Yan, Shirui., Wang, Yun., Zhang, Haoling., .. & Fu, Xian. (2023). A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid. Nature Communications, 14(1).
MLA Chang, Tiantian,et al."A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid". Nature Communications 14.1(2023).
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