High-throughput single-cell assay for precise measurement of the intrinsic mechanical properties and shape characteristics of red blood cells

doi:10.1039/d3lc00323j

科研成果详情

题名	High-throughput single-cell assay for precise measurement of the intrinsic mechanical properties and shape characteristics of red blood cells
作者	Wei，Qiaodong 1; Xiong，Ying 2; Ma，Yuhang 3; Liu，Deyun 1; Lu，Yunshu 4; Zhang，Shenghong 1; Wang，Xiaolong 1; Huang，Huaxiong 5,6,7; Liu，Yingbin 8; Dao，Ming 9,10; Gong，Xiaobo 1,11
发表日期	2023-12-01
发表期刊	Lab on a Chip
ISSN/eISSN	1473-0197
卷号	24 期号:2 页码:305-316
摘要	The intrinsic physical and mechanical properties of red blood cells (RBCs), including their geometric and rheological characteristics, can undergo changes in various circulatory and metabolic diseases. However, clinical diagnosis using RBC biophysical phenotypes remains impractical due to the unique biconcave shape, remarkable deformability, and high heterogeneity within different subpopulations. Here, we combine the hydrodynamic mechanisms of fluid-cell interactions in micro circular tubes with a machine learning method to develop a relatively high-throughput microfluidic technology that can accurately measure the shear modulus of the membrane, viscosity, surface area, and volume of individual RBCs. The present method can detect the subtle changes of mechanical properties in various RBC components at continuum scales in response to different doses of cytoskeletal drugs. We also investigate the correlation between glycosylated hemoglobin and RBC mechanical properties. Our study develops a methodology that combines microfluidic technology and machine learning to explore the material properties of cells based on fluid-cell interactions. This approach holds promise in offering novel label-free single-cell-assay-based biophysical markers for RBCs, thereby enhancing the potential for more robust disease diagnosis.
DOI	10.1039/d3lc00323j
URL	查看来源
语种	英语English
Scopus入藏号	2-s2.0-85179815026
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/11557
专题	北师香港浸会大学
通讯作者	Dao，Ming
作者单位	1.Key Laboratory of Hydrodynamics (Ministry of Education),Department of Engineering Mechanics,School of Naval Architecture Ocean and Civil Engineering,Shanghai Jiao Tong University,Shanghai,200240,China 2.Obstetrics and Gynecology Department,Xinhua Hospital Affiliated to Shanghai Jiao Tong University Medical School,Shanghai,200240,China 3.Endocrinology Department,Shanghai General Hospital,Shanghai,200240,China 4.Department of Breast Surgery,Xinhua Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai,200433,China 5.Research Center for Mathematics,Advanced Institute of Natural Sciences,Beijing Normal University,Zhuhai,Guangdong,519088,China 6.Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science,BNU-HKBU United International College,Zhuhai,Guangdong,519088,China 7.Department of Mathematics and Statistics York University,Toronto,M3J 1P3,Canada 8.Department of Biliary-Pancreatic Surgery,Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai,China 9.Department of Materials Science and Engineering,Massachusetts Institute of Technology,Cambridge,02139,United States 10.School of Biological Sciences,Nanyang Technological University,637551,Singapore 11.State Key Laboratory of Ocean Engineering,School of Naval Architecture,Ocean and Civil Engineering,Shanghai Jiao Tong University,Shanghai,200240,China
推荐引用方式 GB/T 7714	Wei，Qiaodong,Xiong，Ying,Ma，Yuhanget al. High-throughput single-cell assay for precise measurement of the intrinsic mechanical properties and shape characteristics of red blood cells[J]. Lab on a Chip, 2023, 24(2): 305-316.
APA	Wei，Qiaodong., Xiong，Ying., Ma，Yuhang., Liu，Deyun., Lu，Yunshu., .. & Gong，Xiaobo. (2023). High-throughput single-cell assay for precise measurement of the intrinsic mechanical properties and shape characteristics of red blood cells. Lab on a Chip, 24(2), 305-316.
MLA	Wei，Qiaodong,et al."High-throughput single-cell assay for precise measurement of the intrinsic mechanical properties and shape characteristics of red blood cells". Lab on a Chip 24.2(2023): 305-316.