Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii

doi:10.1111/j.1600-079X.1998.tb00528.x

科研成果详情

题名	Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii
作者	Tsim，Siu Tai 1; Wong，Joseph T.Y.1; Wong，Yung H.1,2
发表日期	1998
发表期刊	Journal of Pineal Research
ISSN/eISSN	0742-3098
卷号	24 期号:3 页码:152-161
摘要	Exogenous indoleamines such as melatonin and 5-methoxytryptamine have been shown to induce cyst formation (encystment) in many species of dinoflagellate. Induction of inositol phosphates formation by indoleamine has previously been demonstrated in Crypthecodinium cohnii. In addition, depletion of extracellular Ca blocks the indoleamine-induced encystment. In the present study, 12 indoleamines (including melatonin and related compounds) were examined for their abilities to induce Ca influx, inositol phosphates formation, and encystment in C. cohnii. The results showed that melatonin, 5-methoxytryptamine, and the peptide toxin mastoparan stimulated Ca influxes in dose- and time-dependent manners. The EC values of 5-methoxytrypramine and mastoparan to stimulate Ca uptake were 2 mM and 35 μM, respectively. The 5-methoxytryptamine- and mastoparan-induced Ca influx were partially attenuated by the calcium channel blockers, verapamil and ruthenium red. A series of indoleamines were examined for their structure-activity relationship on the induction of encystment and formation of inositol phosphates. Melatonin-induced inositol phosphates formation was completely blocked by U73122, indicating the possible involvement of phospholipase C. Taken together, we conclude that indoleamines may induce encystment of the dinoflagellate C. cohnii via parallel activation of phospholipase C and Ca influx signaling pathways. However, activation of phospholipase C and Ca influx are not always necessary or sufficient for inducing encystment. Also, these data provided the first direct evidence of a Ca influx regulating mechanism in dinoflagellate C. cohnii.
关键词	5-Methoxytryptamine Calcium influx Dinoflagellates Encystment Mastoparan Melatonin Phospholipase C Structure-activity relationship
DOI	10.1111/j.1600-079X.1998.tb00528.x
URL	查看来源
语种	英语English
Scopus入藏号	2-s2.0-0031971147
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/11705
专题	个人在本单位外知识产出
作者单位	1.Department of Biology,Hong Kong Univ. of Sci. and Technol.,Clear Water Bay,Kowloon,Hong Kong 2.Biotechnology Research Institute,Hong Kong Univ. of Sci. and Technol.,Clear Water Bay,Kowloon,Hong Kong
推荐引用方式 GB/T 7714	Tsim，Siu Tai,Wong，Joseph T.Y.,Wong，Yung H. Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii[J]. Journal of Pineal Research, 1998, 24(3): 152-161.
APA	Tsim，Siu Tai, Wong，Joseph T.Y., & Wong，Yung H. (1998). Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii. Journal of Pineal Research, 24(3), 152-161.
MLA	Tsim，Siu Tai,et al."Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii". Journal of Pineal Research 24.3(1998): 152-161.