Calcium ion dependency and the role of inositol phosphates in melatonin-induced encystment of dinoflagellates

科研成果详情

题名	Calcium ion dependency and the role of inositol phosphates in melatonin-induced encystment of dinoflagellates
作者	Tsim，Siu Tai 1; Wong，Joseph T.Y.1; Wong，Yung H.1,2
发表日期	1997-06-01
发表期刊	Journal of Cell Science
ISSN/eISSN	0021-9533
卷号	110 期号:12 页码:1387-1393
摘要	The unicellular eukaryotic dinoflagellates shed their flagella and form a new pellicle cyst wall in response to environmental stress, This encystment process can also be induced by indoleamines such as melatonin and 5-methoxytryptamine. To decipher the complex signaling events which lead to encystment, we have investigated the functional roles of Ca and inositol phosphates in indoleamine-induced encystment of the dinoflagellates Alexandrium catenella and Crypthecodinium cohnii. Pretreatment with EGTA, but not with EDTA, effectively blocked the indoleamine-induced encystment of A. catenella in a dose-dependent manner. Conversely, agents that facilitate the influx of Ca (Bay K 8644, A23187 and ionomycin) dose-dependently induced encystment of A. catenella. Endoplasmic Ca-ATPase inhibitors such as thapsigargin and the peptide toxin melittin also induced encystment of A. catenella. These results suggest that an elevation of intracellular [Ca] may be involved in the encystment response. In terms of the regulation of phospholipase C, melatonin dose- and time-dependently stimulated the formation of inositol phosphates in C. cohnii. The rank order of potency for several indoleamines to stimulate inositol phosphates formation was 2-iodomelatonin > 5-methoxytryptamine ≤ melatonin >> N-acetylserotonin > 5-hydroxytryptamine. This rank order was the same as for the indoleamine-induced encystment of C. cohnii as previously reported. Our results indicate that indoleamine-induced activation of phospholipase C and elevation of intracellular [Ca] may be proximal steps in the signal transduction pathway leading to encystment in dinoflagellates. Moreover, this is the first demonstration of the possible involvement of Ca and inositol phosphates as second messengers in dinoflagellates.
关键词	5-Methoxytryptamine Calcium Dinoflagellate Encystment Inositol phosphate Melatonin
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语种	英语English
Scopus入藏号	2-s2.0-0030855744
引用统计
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/11706
专题	个人在本单位外知识产出
通讯作者	Wong，Yung H.
作者单位	1.Department of Biology,Hong Kong University of Science and Technology,Kowloon,Clear Water Bay,Hong Kong 2.Biotechnology Research Institute,Hong Kong University of Science and Technology,Kowloon,Clear Water Bay,Hong Kong
推荐引用方式 GB/T 7714	Tsim，Siu Tai,Wong，Joseph T.Y.,Wong，Yung H. Calcium ion dependency and the role of inositol phosphates in melatonin-induced encystment of dinoflagellates[J]. Journal of Cell Science, 1997, 110(12): 1387-1393.
APA	Tsim，Siu Tai, Wong，Joseph T.Y., & Wong，Yung H. (1997). Calcium ion dependency and the role of inositol phosphates in melatonin-induced encystment of dinoflagellates. Journal of Cell Science, 110(12), 1387-1393.
MLA	Tsim，Siu Tai,et al."Calcium ion dependency and the role of inositol phosphates in melatonin-induced encystment of dinoflagellates". Journal of Cell Science 110.12(1997): 1387-1393.