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题名MiR-23b-3p Improves Brain Damage after Status Epilepticus by Reducing the Formation of Pathological High-Frequency Oscillations via Inhibition of cx43 in Rat Hippocampus
作者
发表日期2024-07-17
发表期刊ACS Chemical Neuroscience
ISSN/eISSN1948-7193
卷号15期号:14页码:2633-2642
摘要

In order to investigate the effectiveness and safety of miR-23b-3p in anti-seizure activity and to elucidate the regulatory relationship between miR-23b-3p and Cx43 in the nervous system, we have established a lithium chloride-pilocarpine (PILO) status epilepticus (SE) model. Rats were randomly divided into the following groups: seizure control (PILO), valproate sodium (VPA+PILO), recombinant miR-23b-3p overexpression (miR+PILO), miR-23b-3p sponges (Sponges+PILO), and scramble sequence negative control (Scramble+PILO) (n = 6/group). After experiments, we got the following results. In the acute phase, the time required for rats to reach stage IV after PILO injection was significantly longer in VPA+PILO and miR+PILO. In the chronic phase after SE, the frequency of spontaneous recurrent seizures (SRSs) in VPA+PILO and miR+PILO was significantly reduced. At 10 min before seizure cessation, the average energy expression of fast ripples (FRs) in VPA+PILO and miR+PILO was significantly lower than in PILO. After 28 days of seizure, Cx43 expression in PILO was significantly increased, and Beclin1expression in all groups was significantly increased. After 28 days of SE,the number of synapses in the CA1 region of the hippocampus was significantly higher in the VPA+PILO and miR+PILO groups compared to that in the PILO group. After 28 days of SE ,hippocampal necrotic cells in the CA3 region were significantly lower in the VPA+PILO and miR+PILO groups compared to those in the PILO group. There were no significant differences in biochemical indicators among the experimental group rats 28 days after SE compared to the seizure control group. Based on the previous facts, we can reach the conclusion that MiR-23b-3p targets and blocks the expression of hippocampal Cx43 which can reduce the formation of pathological FRs, thereby alleviating the severity of seizures, improving seizure-induced brain damage.

关键词epilepsy Fast ripples gap junctions hippocampus miR-23b-3p valproic acid
DOI10.1021/acschemneuro.4c00112
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收录类别SCIE
语种英语English
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Neurosciences & Neurology
WOS类目Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Neurosciences
WOS记录号WOS:001265056100001
Scopus入藏号2-s2.0-85197789236
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://repository.uic.edu.cn/handle/39GCC9TT/11740
专题理工科技学院
通讯作者Hu, Yue
作者单位
1.Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Neurol, Minist Educ,Key Lab Child Dev & Disorders,Chongqin, Chongqing 400014, Peoples R China
2.Pediatric Research Institute,Children’s Hospital of Chongqing Medical University,Chongqing,400014,China
3.Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science,BNU-HKBU United International College,Zhuhai,519087,China
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Yi, Yanjun,Zhang, Shimin,Dai, Jialiet al. MiR-23b-3p Improves Brain Damage after Status Epilepticus by Reducing the Formation of Pathological High-Frequency Oscillations via Inhibition of cx43 in Rat Hippocampus[J]. ACS Chemical Neuroscience, 2024, 15(14): 2633-2642.
APA Yi, Yanjun., Zhang, Shimin., Dai, Jiali., Zheng, Hao., Peng, Xiaoling., .. & Hu, Yue. (2024). MiR-23b-3p Improves Brain Damage after Status Epilepticus by Reducing the Formation of Pathological High-Frequency Oscillations via Inhibition of cx43 in Rat Hippocampus. ACS Chemical Neuroscience, 15(14), 2633-2642.
MLA Yi, Yanjun,et al."MiR-23b-3p Improves Brain Damage after Status Epilepticus by Reducing the Formation of Pathological High-Frequency Oscillations via Inhibition of cx43 in Rat Hippocampus". ACS Chemical Neuroscience 15.14(2024): 2633-2642.
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