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题名Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells
作者
发表日期2017
发表期刊Molecular and Cellular Biochemistry
ISSN/eISSN0300-8177
卷号424期号:1-2页码:163-172
摘要

Consumption of fruits and vegetables is generally regarded as beneficial to plasma lipid profile. The mechanism by which the plant foods induce desirable lipid changes remains unclear. SREBP-2 is crucial in cholesterol metabolism, and it is a major regulator of the cholesterol biosynthesis enzyme HMGCR. Our lab has previously illustrated that apigenin and luteolin could attenuate the nuclear translocation of SREBP-2 through an AMPK-dependent pathway. In the present study, these two flavones were studied for their ability to deter the same in an AMPK-independent signaling route. The processing of SREBP-2 protein was promoted by phorbol 12-myristate 13-acetate (PMA) in the hepatic cells WRL and HepG2, and the increased processing was reversed by apigenin or luteolin co-administration. EMSA results demonstrated that the PMA-induced DNA-binding activity was weakened by the flavones. The increased amount of nuclear SREBP-2 in cells was attenuated by the flavonoid as shown by immunocytochemical imaging. Quantitative reverse transcriptase-polymerase chain reaction assay demonstrated that the transcription of HMGCR under both flavone treatments was reduced. However, apigenin appeared to be stronger than luteolin in restraining PMA-induced HMGCR mRNA expression. Since PMA is a diacylglycerol analog, these findings might have some physiological implications.

关键词Apigenin HMGCR Luteolin Nuclear translocation PKC-dependent pathway SREBP-2
DOI10.1007/s11010-016-2851-6
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收录类别SCIE
语种英语English
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000392107800013
Scopus入藏号2-s2.0-84992184013
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://repository.uic.edu.cn/handle/39GCC9TT/13176
专题个人在本单位外知识产出
通讯作者Leung, Lai K.
作者单位
1.Food and Nutritional Sciences Programme,School of Life Sciences,Faculty of Science,The Chinese University of Hong Kong,Shatin,Hong Kong
2.Biochemistry Programmes,School of Life Sciences,Faculty of Science,The Chinese University of Hong Kong,Shatin,Hong Kong
3.Department of Food Science,National Chiayi University,Chiayi City, Taiwan,Taiwan
4.Food and Nutritional Sciences/Biochemistry Programme,School of Life Sciences,The Chinese University of Hong Kong,Shatin, N.T.,Rm.507C MMW Bldg,Hong Kong
推荐引用方式
GB/T 7714
Tan, Yan Qin,Wong, Tsz Yan,Lin, Shu meiet al. Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells[J]. Molecular and Cellular Biochemistry, 2017, 424(1-2): 163-172.
APA Tan, Yan Qin, Wong, Tsz Yan, Lin, Shu mei, & Leung, Lai K. (2017). Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells. Molecular and Cellular Biochemistry, 424(1-2), 163-172.
MLA Tan, Yan Qin,et al."Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells". Molecular and Cellular Biochemistry 424.1-2(2017): 163-172.
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