发表状态 | 已发表Published |
题名 | Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells |
作者 | |
发表日期 | 2017 |
发表期刊 | Molecular and Cellular Biochemistry
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ISSN/eISSN | 0300-8177 |
卷号 | 424期号:1-2页码:163-172 |
摘要 | Consumption of fruits and vegetables is generally regarded as beneficial to plasma lipid profile. The mechanism by which the plant foods induce desirable lipid changes remains unclear. SREBP-2 is crucial in cholesterol metabolism, and it is a major regulator of the cholesterol biosynthesis enzyme HMGCR. Our lab has previously illustrated that apigenin and luteolin could attenuate the nuclear translocation of SREBP-2 through an AMPK-dependent pathway. In the present study, these two flavones were studied for their ability to deter the same in an AMPK-independent signaling route. The processing of SREBP-2 protein was promoted by phorbol 12-myristate 13-acetate (PMA) in the hepatic cells WRL and HepG2, and the increased processing was reversed by apigenin or luteolin co-administration. EMSA results demonstrated that the PMA-induced DNA-binding activity was weakened by the flavones. The increased amount of nuclear SREBP-2 in cells was attenuated by the flavonoid as shown by immunocytochemical imaging. Quantitative reverse transcriptase-polymerase chain reaction assay demonstrated that the transcription of HMGCR under both flavone treatments was reduced. However, apigenin appeared to be stronger than luteolin in restraining PMA-induced HMGCR mRNA expression. Since PMA is a diacylglycerol analog, these findings might have some physiological implications. |
关键词 | Apigenin HMGCR Luteolin Nuclear translocation PKC-dependent pathway SREBP-2 |
DOI | 10.1007/s11010-016-2851-6 |
URL | 查看来源 |
收录类别 | SCIE |
语种 | 英语English |
WOS研究方向 | Cell Biology |
WOS类目 | Cell Biology |
WOS记录号 | WOS:000392107800013 |
Scopus入藏号 | 2-s2.0-84992184013 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://repository.uic.edu.cn/handle/39GCC9TT/13176 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Leung, Lai K. |
作者单位 | 1.Food and Nutritional Sciences Programme,School of Life Sciences,Faculty of Science,The Chinese University of Hong Kong,Shatin,Hong Kong 2.Biochemistry Programmes,School of Life Sciences,Faculty of Science,The Chinese University of Hong Kong,Shatin,Hong Kong 3.Department of Food Science,National Chiayi University,Chiayi City, Taiwan,Taiwan 4.Food and Nutritional Sciences/Biochemistry Programme,School of Life Sciences,The Chinese University of Hong Kong,Shatin, N.T.,Rm.507C MMW Bldg,Hong Kong |
推荐引用方式 GB/T 7714 | Tan, Yan Qin,Wong, Tsz Yan,Lin, Shu meiet al. Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells[J]. Molecular and Cellular Biochemistry, 2017, 424(1-2): 163-172. |
APA | Tan, Yan Qin, Wong, Tsz Yan, Lin, Shu mei, & Leung, Lai K. (2017). Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells. Molecular and Cellular Biochemistry, 424(1-2), 163-172. |
MLA | Tan, Yan Qin,et al."Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells". Molecular and Cellular Biochemistry 424.1-2(2017): 163-172. |
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