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题名Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro
作者
发表日期2020-03-25
发表期刊Journal of Ethnopharmacology
ISSN/eISSN0378-8741
卷号250
摘要

Ethnopharmacological relevance: Sigesbeckiae Herba (SH), a traditional anti-inflammatory Chinese herbal medicine, is originated from the plants of Sigesbeckia pubescens Makino (SP), S. orientalis L. (SO) and S. glabrescens Makino (SG). The current studies reported that the chemical constituents in the three species of plants were different. Aim of the study: The aim of this study is to provide a systemic comparison on the anti-inflammatory effects and the underlying molecular mechanisms among the three plants based on their effects on nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs) signal pathways in vitro. Material and methods: Twenty-four batches of three Sigesbeckia herbs were collected from different regions of China and extracted with 50% ethanol. The distribution of 6 compounds in the 24 batches of SH extracts were characterized by UPLC analysis. The cytotoxicity of all extracts to RAW264.7 cells in the absence or presence of lipopolysaccharide (LPS) were examined by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-inflammatory effects of the extracts were investigated using Griess reagent and enzyme-linked immunosorbent assay. The underlying mechanisms of the representative samples (SP007, SO005 and SG003) for individual species were examined by western blotting and immunofluorescence staining. Results: The estimated average sub-lethal dose (LD) of SP, SO and SG on RAW264.7 cells were 181.7 ± 15.7, 291.5 ± 33.9 and 317.1 ± 16.3 μg/mL, respectively. In LPS-stimulated RAW264.7 cells, the inhibitory effects of SH species were determined to be SP > SO > SG on NO release, while SP ~ SO > SG on secretion of post-inflammatory cytokines (TNF-α, IL-6 and MCP-1). Moreover, suppression on LPS-induced excessive expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), as well as the activation of NF-κB and phosphorylation of MAPKs were investigated to be associated to the anti-inflammatory effects for all SH species. Conclusions: We firstly reported a systemic comparison on the anti-inflammatory properties for the three main plant origins of SH. Although SG showed lower toxicity and less anti-inflammatory effects compared with SP and SO in LPS-induced RAW264.7 cells, comparable inhibitory effects on NF-κB and MAPKs pathways and the reduction of LPS-induced iNOS and COX-2 were observed in the anti-inflammatory process for all Sigesbeckia plants.

关键词Anti-inflammation MAPKs NF-κB Sigesbeckia glabrescens makino Sigesbeckia orientalis L. Sigesbeckia pubescens makino
DOI10.1016/j.jep.2019.112530
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收录类别SCIE
语种英语English
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号WOS:000510954100042
Scopus入藏号2-s2.0-85076851841
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://repository.uic.edu.cn/handle/39GCC9TT/6184
专题北师香港浸会大学
通讯作者Yu, Hua
作者单位
1.Institute of Chinese Medical Sciences,State Key Laboratory of Quality Research in Chinese Medicine,University of Macau,China
2.HKBU Shenzhen Research Center,Shenzhen,China
3.School of Chinese Medicine,Hong Kong Baptist University,Kowloon Tong,Hong Kong
4.Programme of Food Science and Technology,Division of Science and Technology,Beijing Normal University-Hong Kong Baptist University United International College,Zhuhai,China
推荐引用方式
GB/T 7714
Linghu, Ke Gang,Zhao, Guan Ding,Xiong, Weiet al. Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro[J]. Journal of Ethnopharmacology, 2020, 250.
APA Linghu, Ke Gang., Zhao, Guan Ding., Xiong, Wei., Sang, Wei., Xiong, Shi Hang., .. & Yu, Hua. (2020). Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro. Journal of Ethnopharmacology, 250.
MLA Linghu, Ke Gang,et al."Comprehensive comparison on the anti-inflammatory effects of three species of Sigesbeckia plants based on NF-κB and MAPKs signal pathways in vitro". Journal of Ethnopharmacology 250(2020).
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