A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells

doi:10.7150/ijms.15449

科研成果详情

题名	A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells
作者	Wang，Ruihua 1; MoYung，K. C.2; Zhao，Y. J.2; Poon，Karen 2
发表日期	2016-06-29
发表期刊	International Journal of Medical Sciences
ISSN/eISSN	1449-1907
卷号	13 期号:7 页码:507-516
摘要	OBJECTIVES: To investigate the potentiation effect of Genipin to Cisplatin induced cell senescence in HCT-116 colon cancer cells in vitro. METHODS: Cell viability was estimated by Propidium iodide and Hoechst 3342, reactive oxygen species (ROS) with DHE, mitochondrial membrane potential (MMP) with JC-1 MMP assay Kit and electron current production with microbial fuel cells (MFC). RESULTS: Genipin inhibited the UCP2 mediated anti-oxidative proton leak significantly promoted the Cisplatin induced ROS and subsequent cell death, which was similar to that of UCP2-siRNA. Cells treated with Cisplatin alone or combined with Genipin, ROS negatively, while MMP positively correlated with cell viability. Cisplatin induced ROS was significantly decreased by detouring electrons to MFC, or increased by Genipin combined treatment. Compensatory effects of UCP2 up-regulation with time against Genipin treatment were suggested. Shorter the Genipin treatment before Cisplatin better promoted the Cisplatin induced ROS and subsequent cell death. CONCLUSION: The interaction of leaked electron with Cisplatin was important during ROS generation. Inhibition of UCP2-mediated proton leak with Genipin potentiated the cytotoxicity of Cisplatin. Owing to the compensatory effects against Genipin, shorter Genipin treatment before Cisplatin was recommended in order to achieve better potentiation effect.
关键词	Cell electric current Cisplatin Cytotoxicity Genipin Mitochondrial membrane potential Reactive oxygen species
DOI	10.7150/ijms.15449
URL	查看来源
语种	英语English
Scopus入藏号	2-s2.0-84979053863
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/6403
专题	北师香港浸会大学
通讯作者	Poon，Karen
作者单位	1.Department of Gastroenterology,Shenzhen Hospital of Southern Medical University,Shenzhen,518100,China 2.Division of Science and Technology,BNU-HKBU United International College,Tangjiawan, Zhuhai,28 Jinfeng Road,519085,China
通讯作者单位	北师香港浸会大学
推荐引用方式 GB/T 7714	Wang，Ruihua,MoYung，K. C.,Zhao，Y. J.et al. A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells[J]. International Journal of Medical Sciences, 2016, 13(7): 507-516.
APA	Wang，Ruihua, MoYung，K. C., Zhao，Y. J., & Poon，Karen. (2016). A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells. International Journal of Medical Sciences, 13(7), 507-516.
MLA	Wang，Ruihua,et al."A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells". International Journal of Medical Sciences 13.7(2016): 507-516.