UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties

doi:10.1007/s11356-015-5155-6

科研成果详情

题名	UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties
作者	Wang，Ruihua 2; MoYung，K. C.1; Zhang，M. H.1; Poon，Karen 1
发表日期	2015-12-01
发表期刊	Environmental Science and Pollution Research
ISSN/eISSN	0944-1344
卷号	22 期号:24 页码:19618-19631
摘要	Using live eukaryotic cells, including cancer cells, MCF-7 and HCT-116, normal hepatocytes and red blood cells in anode and potassium ferricyanide in cathode of MFC could generate bio-based electric current. Electrons and protons generated from the metabolic reaction in both cytosol and mitochondria contributing to the leaking would mediate the generation of electric current. Both resveratrol (RVT) and 2,4-dinitrophenol (DNP) used to induce proton leak in mitochondria were found to promote electric current production in all cells except red blood cells without mitochondria. Proton leak might be important for electric current production by bringing the charge balance in cells to enhance the further electron leak. The induced electric current by RVT can be blocked by Genipin, an inhibitor of UCP2-mediated proton leak, while that induced by DNP cannot. RVT could reduce reactive oxygen species (ROS) level in cells better than that of DNP. In addition, RVT increased mitochondrial membrane potential (MMP), while DNP decreased it. Results highly suggested the existence of at least two types of electric current that showed different properties. They included UCP2-mediated and non-UCP2-mediated electric current. UCP2-mediated electric current exhibited higher reactive oxygen species (ROS) reduction effect per unit electric current production than that of non-UCP2-mediated electric current. Higher UCP2-mediated electric current observed in cancer cells might contribute to the mechanism of drug resistence. Correlation could not be established between electric current production with either ROS and MMP without distinguishing the types of electric current.
关键词	2,4-Dinitrophenol Cellular electric current Electron and proton leak Genipin Microbial fuel cell Resveratrol
DOI	10.1007/s11356-015-5155-6
URL	查看来源
语种	英语English
Scopus入藏号	2-s2.0-84951568137
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/6434
专题	北师香港浸会大学
通讯作者	Poon，Karen
作者单位	1.Program of Food Science and Technology,Division of Science and Technology,BNU-HKBU United International College,Zhuhai,28 Jinfeng Road, Tangjiawan,519085,China 2.Department of Gastroenterology,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital South Campus,Shanghai,6600 Nanfeng Road, Fengxian District,201499,China
通讯作者单位	北师香港浸会大学
推荐引用方式 GB/T 7714	Wang，Ruihua,MoYung，K. C.,Zhang，M. H.et al. UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties[J]. Environmental Science and Pollution Research, 2015, 22(24): 19618-19631.
APA	Wang，Ruihua, MoYung，K. C., Zhang，M. H., & Poon，Karen. (2015). UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties. Environmental Science and Pollution Research, 22(24), 19618-19631.
MLA	Wang，Ruihua,et al."UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties". Environmental Science and Pollution Research 22.24(2015): 19618-19631.