Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters

doi:10.1039/c1ay05347g

科研成果详情

题名	Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters
作者	Lei，Hongtao 1; Xue，Gang 1; Yu，Chunfai 2; Haughey，Simon A.3; Eremin，Sergei A.4; Sun，Yuanming 1; Wang，Zhanhui 5; Xu，Zhenlin 1; Wang，Hong 1; Shen，Yudong 1; Wu，Qing 1
发表日期	2011
发表期刊	Analytical Methods
ISSN/eISSN	1759-9660
卷号	3 期号:10 页码:2334-2340
摘要	A rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for the detection of a widely used herbicide in rice cultivation, butachlor, in various polluted water samples. Three fluorescent tracers with different spacer arms and structures were synthesized to enhance the FPIA sensitivity. The effects of different fluorescent tracer structure and organic solvent concentration on the performance of the FPIA were investigated. The results showed that the fluorescent tracer 3-(2-((butoxymethyl)(2,6- diethylphenyl)amino)-2-oxoethylthio)-N-(6-(3-(3′,6′-dihydroxy-3-oxo- 3H-spiro[isobenzofuran-1,9′-xanthene]-5-yl) thioureido)hexylpropanamide (tracer C) demonstrated the best FPIA sensitivity at pH 8.0, which remained at up to 20% methanol in a tolerance study. The IC (50% inhibition concentration) of the FPIA was 207.0 ng mL with the detection of butachlor ranging from 32.0 to 1218.0 ng mL (at 20%-80% inhibition concentration), and the limit of detection (at 10% inhibition concentration) was 11.0 ng mL. Recoveries, measured in spiked water samples, ranged from 75.1% to 134.5%. This newly developed FPIA was characterized by high specificity and reproducibility, allowing a rapid, low-cost, and sensitive determination of butachlor in polluted waters. © 2011 The Royal Society of Chemistry.
DOI	10.1039/c1ay05347g
URL	查看来源
语种	英语English
Scopus入藏号	2-s2.0-80052206592
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/6606
专题	北师香港浸会大学
通讯作者	Wu，Qing
作者单位	1.Guangdong Provincial Key Laboratory of Food Quality and Safety,South China Agricultural University,Guangzhou 510642,China 2.Environmental Science Program,United International College,Zhuhai 519085,China 3.Institute of Agri-Food and Land Use,Queen's University Belfast,Belfast BT9 5AG,United Kingdom 4.Department of Chemical Enzymology,Faculty of Chemistry,M.V. Lomonosov Moscow State University,Moscow 119998,Russian Federation 5.Department of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,China Agricultural University,Beijing 100094,China
推荐引用方式 GB/T 7714	Lei，Hongtao,Xue，Gang,Yu，Chunfaiet al. Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters[J]. Analytical Methods, 2011, 3(10): 2334-2340.
APA	Lei，Hongtao., Xue，Gang., Yu，Chunfai., Haughey，Simon A., Eremin，Sergei A., .. & Wu，Qing. (2011). Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters. Analytical Methods, 3(10), 2334-2340.
MLA	Lei，Hongtao,et al."Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters". Analytical Methods 3.10(2011): 2334-2340.