| Title | Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii |
| Creator | |
| Date Issued | 1998 |
| Source Publication | Journal of Pineal Research
 |
| ISSN | 0742-3098 |
| Volume | 24Issue:3Pages:152-161 |
| Abstract | Exogenous indoleamines such as melatonin and 5-methoxytryptamine have been shown to induce cyst formation (encystment) in many species of dinoflagellate. Induction of inositol phosphates formation by indoleamine has previously been demonstrated in Crypthecodinium cohnii. In addition, depletion of extracellular Ca blocks the indoleamine-induced encystment. In the present study, 12 indoleamines (including melatonin and related compounds) were examined for their abilities to induce Ca influx, inositol phosphates formation, and encystment in C. cohnii. The results showed that melatonin, 5-methoxytryptamine, and the peptide toxin mastoparan stimulated Ca influxes in dose- and time-dependent manners. The EC values of 5-methoxytrypramine and mastoparan to stimulate Ca uptake were 2 mM and 35 μM, respectively. The 5-methoxytryptamine- and mastoparan-induced Ca influx were partially attenuated by the calcium channel blockers, verapamil and ruthenium red. A series of indoleamines were examined for their structure-activity relationship on the induction of encystment and formation of inositol phosphates. Melatonin-induced inositol phosphates formation was completely blocked by U73122, indicating the possible involvement of phospholipase C. Taken together, we conclude that indoleamines may induce encystment of the dinoflagellate C. cohnii via parallel activation of phospholipase C and Ca influx signaling pathways. However, activation of phospholipase C and Ca influx are not always necessary or sufficient for inducing encystment. Also, these data provided the first direct evidence of a Ca influx regulating mechanism in dinoflagellate C. cohnii. |
| Keyword | 5-Methoxytryptamine Calcium influx Dinoflagellates Encystment Mastoparan Melatonin Phospholipase C Structure-activity relationship |
| DOI | 10.1111/j.1600-079X.1998.tb00528.x |
| URL | View source |
| Language | 英语English |
| Scopus ID | 2-s2.0-0031971147 |
| Citation statistics | |
| Document Type | Journal article |
| Identifier | http://repository.uic.edu.cn/handle/39GCC9TT/11705 |
| Collection | Research outside affiliated institution |
| Affiliation | 1.Department of Biology,Hong Kong Univ. of Sci. and Technol.,Clear Water Bay,Kowloon,Hong Kong 2.Biotechnology Research Institute,Hong Kong Univ. of Sci. and Technol.,Clear Water Bay,Kowloon,Hong Kong |
| Recommended Citation GB/T 7714 | Tsim，Siu Tai,Wong，Joseph T.Y.,Wong，Yung H. Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii[J]. Journal of Pineal Research, 1998, 24(3): 152-161. |
| APA | Tsim，Siu Tai, Wong，Joseph T.Y., & Wong，Yung H. (1998). Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii. Journal of Pineal Research, 24(3), 152-161. |
| MLA | Tsim，Siu Tai,et al."Regulation of calcium influx and phospholipase C activity by indoleamines in dinoflagellate Crypthecodinium cohnii". Journal of Pineal Research 24.3(1998): 152-161. |
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