| Title | At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea (Ampelopsis grossedentata) |
| Creator | |
| Date Issued | 2017 |
| Source Publication | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
 |
| ISSN | 1570-0232 |
| Volume | 1040Pages:112-117 |
| Abstract | Vine tea (Ampelopsis grossedentata), a widely used healthy tea, beverage and herbal medicine, exhibited strong antioxidant activity. However, systematic purification of antioxidants, especially for those with similar structures or polarities, is a challenging work. Here, we present a novel at-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography (HSCCC-Sephadex LH-20 CC) for rapid and efficient separation of antioxidants from vine tea target-guided by 1,1-diphenyl-2-picryl-hydrazyl radical-high performance liquid chromatography (DPPH-HPLC) experiment. A makeup pump, a six-port switching valve and a trapping column were served as interface. The configuration had no operational time and mobile phase limitations between two dimensional chromatography and showed great flexibility without tedious sample-handling procedure. Seven targeted antioxidants were firstly separated by stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems, and then co-eluted antioxidants were on-line trapped, concentrated and desorbed to Sephadex LH-20 column for further off-line purification by methanol. It is noted that six elucidated antioxidants with purity over 95% exhibited stronger activity than ascorbic acid (VC). More importantly, this at-line hyphenated strategy could sever as a rapid and efficient pathway for systematic purification of bioactive components from complex matrix. |
| Keyword | Antioxidant At-line hyphenation HSCCC Sephadex LH-20 CC Vine tea |
| DOI | 10.1016/j.jchromb.2016.11.037 |
| URL | View source |
| Language | 英语English |
| Scopus ID | 2-s2.0-85004073537 |
| Citation statistics | |
| Document Type | Journal article |
| Identifier | http://repository.uic.edu.cn/handle/39GCC9TT/6387 |
| Collection | Beijing Normal-Hong Kong Baptist University |
| Corresponding Author | Shi，Shuyun |
| Affiliation | 1.College of Chemistry and Chemical Engineering,Central South University,Changsha,410083,China 2.Food Science and Technology Program,Beijing Normal University-Hongkong Baptist University United International College,Zhuhai,519085,China |
| Recommended Citation GB/T 7714 | Ma，Ruyi,Zhou，Rongrong,Tong，Runnaet al. At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea (Ampelopsis grossedentata)[J]. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2017, 1040: 112-117. |
| APA | Ma，Ruyi, Zhou，Rongrong, Tong，Runna, Shi，Shuyun, & Chen，Xiaoqing. (2017). At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea (Ampelopsis grossedentata). Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1040, 112-117. |
| MLA | Ma，Ruyi,et al."At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea (Ampelopsis grossedentata)". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1040(2017): 112-117. |
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