| Title | A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells |
| Creator | |
| Date Issued | 2016-06-29 |
| Source Publication | International Journal of Medical Sciences
 |
| ISSN | 1449-1907 |
| Volume | 13Issue:7Pages:507-516 |
| Abstract | OBJECTIVES: To investigate the potentiation effect of Genipin to Cisplatin induced cell senescence in HCT-116 colon cancer cells in vitro. METHODS: Cell viability was estimated by Propidium iodide and Hoechst 3342, reactive oxygen species (ROS) with DHE, mitochondrial membrane potential (MMP) with JC-1 MMP assay Kit and electron current production with microbial fuel cells (MFC). RESULTS: Genipin inhibited the UCP2 mediated anti-oxidative proton leak significantly promoted the Cisplatin induced ROS and subsequent cell death, which was similar to that of UCP2-siRNA. Cells treated with Cisplatin alone or combined with Genipin, ROS negatively, while MMP positively correlated with cell viability. Cisplatin induced ROS was significantly decreased by detouring electrons to MFC, or increased by Genipin combined treatment. Compensatory effects of UCP2 up-regulation with time against Genipin treatment were suggested. Shorter the Genipin treatment before Cisplatin better promoted the Cisplatin induced ROS and subsequent cell death. CONCLUSION: The interaction of leaked electron with Cisplatin was important during ROS generation. Inhibition of UCP2-mediated proton leak with Genipin potentiated the cytotoxicity of Cisplatin. Owing to the compensatory effects against Genipin, shorter Genipin treatment before Cisplatin was recommended in order to achieve better potentiation effect. |
| Keyword | Cell electric current Cisplatin Cytotoxicity Genipin Mitochondrial membrane potential Reactive oxygen species |
| DOI | 10.7150/ijms.15449 |
| URL | View source |
| Language | 英语English |
| Scopus ID | 2-s2.0-84979053863 |
| Citation statistics | |
| Document Type | Journal article |
| Identifier | http://repository.uic.edu.cn/handle/39GCC9TT/6403 |
| Collection | Beijing Normal-Hong Kong Baptist University |
| Corresponding Author | Poon，Karen |
| Affiliation | 1.Department of Gastroenterology,Shenzhen Hospital of Southern Medical University,Shenzhen,518100,China 2.Division of Science and Technology,BNU-HKBU United International College,Tangjiawan, Zhuhai,28 Jinfeng Road,519085,China |
| Corresponding Author Affilication | Beijing Normal-Hong Kong Baptist University |
| Recommended Citation GB/T 7714 | Wang，Ruihua,MoYung，K. C.,Zhao，Y. J.et al. A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells[J]. International Journal of Medical Sciences, 2016, 13(7): 507-516. |
| APA | Wang，Ruihua, MoYung，K. C., Zhao，Y. J., & Poon，Karen. (2016). A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells. International Journal of Medical Sciences, 13(7), 507-516. |
| MLA | Wang，Ruihua,et al."A mechanism for the temporal potentiation of genipin to the cytotoxicity of cisplatin in colon cancer cells". International Journal of Medical Sciences 13.7(2016): 507-516. |
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