The puzzling issue of 'vehicle-treated control' when using ethanol as drug carrier for MCF-7 cells

doi:10.1002/ptr.5153

科研成果详情

题名	The puzzling issue of 'vehicle-treated control' when using ethanol as drug carrier for MCF-7 cells
作者	Chan，Robbie Yat Kan 1; Lee，Kenneth Ka Ho 2; Wong，Wai Yeung 3; Tang，Johnny Cheuk On 4; Lam，Kim Hung 4; Cheng，Chor Hing 4; Lau，Fung Yi 5; Kok，Stanton Hon Lung 2; Gambari，Roberto 6; Chui，Chung Hin 3,4,5
发表日期	2014
发表期刊	Phytotherapy Research
ISSN/eISSN	0951-418X
卷号	28 期号:11 页码:1735-1736
摘要	Ethanol has been commonly used as a vehicle for drug discovery purpose in vitro. The human breast cancer MCF-7 estrogen dependent cell line is a common in vitro model used for hormonal therapy study. However, special precaution is suggested when ethanol is used in pharmacological tests as solvent in order to evaluate the biological activity of potential drugs especially concerning about the MCF-7. Ethanol was shown to stimulate the proliferation of this estrogen receptor positive cell line. Here, we have further demonstrated that the dose responsive stimulatory effect of ethanol on the MCF-7 cells after pre-incubating the breast carcinoma cells with phenol red-free medium and stripped fetal bovine serum. Our findings open a discussion for the evaluation of ethanol as solvent for drug discovery and screening when using MCF-7 cells as a testing model.
关键词	Ethanol MCF-7 cells Proliferation Vehicle
DOI	10.1002/ptr.5153
URL	查看来源
语种	英语English
Scopus入藏号	2-s2.0-84915815402
引用统计
文献类型	期刊论文
条目标识符	https://repository.uic.edu.cn/handle/39GCC9TT/6513
专题	北师香港浸会大学
作者单位	1.Programme of Food Science and Technology,Division of Science and Technology,Beijing Normal University-Hong Kong Baptist University United International College,Zhuhai,China 2.MOE Key Laboratory for Regenerative Medicine,School of Biomedical Sciences,Chinese University of Hong Kong,Hong Kong,Hong Kong 3.Clinical Division,Department of Chemistry,Hong Kong Baptist University,Hong Kong,Hong Kong 4.Department of Applied Biology and Chemical Technology,Hong Kong Polytechnic University,Hong Kong,Hong Kong 5.Department of Medicine and Therapeutics,Chinese University of Hong Kong,Hong Kong,Hong Kong 6.Department of Life Sciences and Biotechnology,University of Ferrara,Ferrara,Italy
第一作者单位	北师香港浸会大学
推荐引用方式 GB/T 7714	Chan，Robbie Yat Kan,Lee，Kenneth Ka Ho,Wong，Wai Yeunget al. The puzzling issue of 'vehicle-treated control' when using ethanol as drug carrier for MCF-7 cells[J]. Phytotherapy Research, 2014, 28(11): 1735-1736.
APA	Chan，Robbie Yat Kan., Lee，Kenneth Ka Ho., Wong，Wai Yeung., Tang，Johnny Cheuk On., Lam，Kim Hung., .. & Chui，Chung Hin. (2014). The puzzling issue of 'vehicle-treated control' when using ethanol as drug carrier for MCF-7 cells. Phytotherapy Research, 28(11), 1735-1736.
MLA	Chan，Robbie Yat Kan,et al."The puzzling issue of 'vehicle-treated control' when using ethanol as drug carrier for MCF-7 cells". Phytotherapy Research 28.11(2014): 1735-1736.