| Title | Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters |
| Creator | |
| Date Issued | 2011 |
| Source Publication | Analytical Methods
 |
| ISSN | 1759-9660 |
| Volume | 3Issue:10Pages:2334-2340 |
| Abstract | A rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for the detection of a widely used herbicide in rice cultivation, butachlor, in various polluted water samples. Three fluorescent tracers with different spacer arms and structures were synthesized to enhance the FPIA sensitivity. The effects of different fluorescent tracer structure and organic solvent concentration on the performance of the FPIA were investigated. The results showed that the fluorescent tracer 3-(2-((butoxymethyl)(2,6- diethylphenyl)amino)-2-oxoethylthio)-N-(6-(3-(3′,6′-dihydroxy-3-oxo- 3H-spiro[isobenzofuran-1,9′-xanthene]-5-yl) thioureido)hexylpropanamide (tracer C) demonstrated the best FPIA sensitivity at pH 8.0, which remained at up to 20% methanol in a tolerance study. The IC (50% inhibition concentration) of the FPIA was 207.0 ng mL with the detection of butachlor ranging from 32.0 to 1218.0 ng mL (at 20%-80% inhibition concentration), and the limit of detection (at 10% inhibition concentration) was 11.0 ng mL. Recoveries, measured in spiked water samples, ranged from 75.1% to 134.5%. This newly developed FPIA was characterized by high specificity and reproducibility, allowing a rapid, low-cost, and sensitive determination of butachlor in polluted waters. © 2011 The Royal Society of Chemistry. |
| DOI | 10.1039/c1ay05347g |
| URL | View source |
| Language | 英语English |
| Scopus ID | 2-s2.0-80052206592 |
| Citation statistics | |
| Document Type | Journal article |
| Identifier | http://repository.uic.edu.cn/handle/39GCC9TT/6606 |
| Collection | Beijing Normal-Hong Kong Baptist University |
| Corresponding Author | Wu，Qing |
| Affiliation | 1.Guangdong Provincial Key Laboratory of Food Quality and Safety,South China Agricultural University,Guangzhou 510642,China 2.Environmental Science Program,United International College,Zhuhai 519085,China 3.Institute of Agri-Food and Land Use,Queen's University Belfast,Belfast BT9 5AG,United Kingdom 4.Department of Chemical Enzymology,Faculty of Chemistry,M.V. Lomonosov Moscow State University,Moscow 119998,Russian Federation 5.Department of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,China Agricultural University,Beijing 100094,China |
| Recommended Citation GB/T 7714 | Lei，Hongtao,Xue，Gang,Yu，Chunfaiet al. Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters[J]. Analytical Methods, 2011, 3(10): 2334-2340. |
| APA | Lei，Hongtao., Xue，Gang., Yu，Chunfai., Haughey，Simon A., Eremin，Sergei A., .. & Wu，Qing. (2011). Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters. Analytical Methods, 3(10), 2334-2340. |
| MLA | Lei，Hongtao,et al."Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters". Analytical Methods 3.10(2011): 2334-2340. |
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