发表状态 | 已发表Published |
题名 | Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil |
作者 | |
发表日期 | 2018-02-01 |
发表期刊 | 3 Biotech
![]() |
ISSN/eISSN | 2190-572X |
卷号 | 8期号:2 |
摘要 | A novel extracellular enzyme with strong fibrinolytic activity, produced by Bacillus tequilensis, which was isolated from the soil of Zhuhai City (China) was purified and characterized. The enzyme was secreted by cultured B. tequilensis in solid state and purified at a high efficiency using the combination of salting out, ion exchange chromatography, and size exclusion chromatography. The enzyme was estimated to have a molecular weight of approximately 27 kDa, pI of 8.9 ± 0.1, to stable at pH 5.0–12.0 and up to 50 °C; the optimum pH and temperature are 10.5 and 45 °C (2373.59 ± 54.81 U/mg), respectively. The fibrinolytic activity was enhanced by K, Na, Mg, Mn, Ca, and Ba and inhibited by Cu, Zn, and Fe. Moreover, the activity was slightly enhanced by PMSF and EDTA at low concentrations and inhibited by β-mercaptoethanol. The N-terminal amino acid sequence is AQSVPYGISQI. The enzyme has a higher enzymatic activity than most other fibrinolytic enzymes. The high thermal stability indicated that it is easy to preserve and could be activated under high-temperature conditions. |
关键词 | Bacillus tequilensis Characterization Fibrinolytic enzyme Identification Purification |
DOI | 10.1007/s13205-018-1115-4 |
URL | 查看来源 |
收录类别 | SCIE |
语种 | 英语English |
WOS记录号 | WOS:000424104200003 |
Scopus入藏号 | 2-s2.0-85041623603 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://repository.uic.edu.cn/handle/39GCC9TT/6317 |
专题 | 理工科技学院 |
通讯作者 | Lei, Bo |
作者单位 | 1.Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, 28 Jinfeng Road, Tangjiawan, 519085, China 2.State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, 224 Waterloo Road, Hong Kong, China 3.Zhuhai Key Laboratory of Agriculture Product Quality and Food Safety, Zhuhai, 28, Jinfeng Road, Tangjiawan, 519085, China 4.Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research University (Deemed to be University), Guntur, 5222, India |
第一作者单位 | 北师香港浸会大学 |
通讯作者单位 | 北师香港浸会大学 |
推荐引用方式 GB/T 7714 | Xin, Xiong,Ambati, Ranga Rao,Cai, Zongweiet al. Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil[J]. 3 Biotech, 2018, 8(2). |
APA | Xin, Xiong, Ambati, Ranga Rao, Cai, Zongwei, & Lei, Bo. (2018). Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil. 3 Biotech, 8(2). |
MLA | Xin, Xiong,et al."Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil". 3 Biotech 8.2(2018). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论