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题名Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil
作者
发表日期2018-02-01
发表期刊3 Biotech
ISSN/eISSN2190-572X
卷号8期号:2
摘要

A novel extracellular enzyme with strong fibrinolytic activity, produced by Bacillus tequilensis, which was isolated from the soil of Zhuhai City (China) was purified and characterized. The enzyme was secreted by cultured B. tequilensis in solid state and purified at a high efficiency using the combination of salting out, ion exchange chromatography, and size exclusion chromatography. The enzyme was estimated to have a molecular weight of approximately 27 kDa, pI of 8.9 ± 0.1, to stable at pH 5.0–12.0 and up to 50 °C; the optimum pH and temperature are 10.5 and 45 °C (2373.59 ± 54.81 U/mg), respectively. The fibrinolytic activity was enhanced by K, Na, Mg, Mn, Ca, and Ba and inhibited by Cu, Zn, and Fe. Moreover, the activity was slightly enhanced by PMSF and EDTA at low concentrations and inhibited by β-mercaptoethanol. The N-terminal amino acid sequence is AQSVPYGISQI. The enzyme has a higher enzymatic activity than most other fibrinolytic enzymes. The high thermal stability indicated that it is easy to preserve and could be activated under high-temperature conditions.

关键词Bacillus tequilensis Characterization Fibrinolytic enzyme Identification Purification
DOI10.1007/s13205-018-1115-4
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收录类别SCIE
语种英语English
WOS记录号WOS:000424104200003
Scopus入藏号2-s2.0-85041623603
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://repository.uic.edu.cn/handle/39GCC9TT/6317
专题理工科技学院
通讯作者Lei, Bo
作者单位
1.Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, 28 Jinfeng Road, Tangjiawan, 519085, China
2.State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, 224 Waterloo Road, Hong Kong, China
3.Zhuhai Key Laboratory of Agriculture Product Quality and Food Safety, Zhuhai, 28, Jinfeng Road, Tangjiawan, 519085, China
4.Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research University (Deemed to be University), Guntur, 5222, India
第一作者单位北师香港浸会大学
通讯作者单位北师香港浸会大学
推荐引用方式
GB/T 7714
Xin, Xiong,Ambati, Ranga Rao,Cai, Zongweiet al. Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil[J]. 3 Biotech, 2018, 8(2).
APA Xin, Xiong, Ambati, Ranga Rao, Cai, Zongwei, & Lei, Bo. (2018). Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil. 3 Biotech, 8(2).
MLA Xin, Xiong,et al."Purification and characterization of fibrinolytic enzyme from a bacterium isolated from soil". 3 Biotech 8.2(2018).
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