题名 | Epac2-deficiency leads to more severe retinal swelling, glial reactivity and oxidative stress in transient middle cerebral artery occlusion induced ischemic retinopathy |
作者 | |
发表日期 | 2015-06-20 |
发表期刊 | Science China Life Sciences
![]() |
ISSN/eISSN | 1674-7305 |
卷号 | 58期号:6页码:521-530 |
摘要 | Ischemia occurs in diabetic retinopathy with neuronal loss, edema, glial cell reactivity and oxidative stress. Epacs, consisting of Epac1 and Epac2, are cAMP mediators playing important roles in maintenance of endothelial barrier and neuronal functions. To investigate the roles of Epacs in the pathogenesis of ischemic retinopathy, transient middle cerebral artery occlusion (tMCAO) was performed on Epac1-deficient (Epac1-/-) mice, Epac2-deficient (Epac2-/-) mice, and their wild type counterparts (Epac1+/+ and Epac2+/+). Two-hour occlusion and 22-hour reperfusion were conducted to induce ischemia/reperfusion injury to the retina. After tMCAO, the contralateral retinae displayed similar morphology between different genotypes. Neuronal loss, retinal edema and increase in immunoreactivity for aquaporin 4 (AQP4), glial fibrillary acidic protein (GFAP), peroxiredoxin 6 (Prx6) were observed in ipsilateral retinae. Epac2-/- ipsilateral retinae showed more neuronal loss in retinal ganglion cell layer, increased retinal thickness and stronger immunostaining of AQP4, GFAP, and Prx6 than those of Epac2+/+. However, Epac1-/- ipsilateral retinae displayed similar pathology as those in Epac1+/+ mice. Our observations suggest that Epac2-deficiency led to more severe ischemic retinopathy after retinal ischemia/reperfusion injury. |
关键词 | Epac ischemia retina retinopathy |
DOI | 10.1007/s11427-015-4860-1 |
URL | 查看来源 |
语种 | 英语English |
Scopus入藏号 | 2-s2.0-84931566593 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://repository.uic.edu.cn/handle/39GCC9TT/6450 |
专题 | 北师香港浸会大学 |
通讯作者 | Chung,Sookja Kim |
作者单位 | 1.Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong,Hong Kong,China 2.Department of Ophthalmology, Li Ka Shing Faculty of Medicine, The University of Hong Kong,Hong Kong,China 3.Research Centre of Heart, Brain, Hormone and Healthy Aging, Li Ka Shing Faculty of Medicine, The University of Hong Kong,Hong Kong,China 4.Division of Science and Technology, United International College,Zhuhai,519085,China 5.State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong,Hong Kong,China |
推荐引用方式 GB/T 7714 | Liu,Jin,Yeung,Patrick Ka Kit,Cheng,Luet al. Epac2-deficiency leads to more severe retinal swelling, glial reactivity and oxidative stress in transient middle cerebral artery occlusion induced ischemic retinopathy[J]. Science China Life Sciences, 2015, 58(6): 521-530. |
APA | Liu,Jin, Yeung,Patrick Ka Kit, Cheng,Lu, Lo,Amy Cheuk Yin, Chung,Stephen Sum Man, & Chung,Sookja Kim. (2015). Epac2-deficiency leads to more severe retinal swelling, glial reactivity and oxidative stress in transient middle cerebral artery occlusion induced ischemic retinopathy. Science China Life Sciences, 58(6), 521-530. |
MLA | Liu,Jin,et al."Epac2-deficiency leads to more severe retinal swelling, glial reactivity and oxidative stress in transient middle cerebral artery occlusion induced ischemic retinopathy". Science China Life Sciences 58.6(2015): 521-530. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论